Bioanalytics Analytical Methods and Concepts in Biochemistry and Molecular Biology

Description

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids.



The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird’s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists.

The following techniques are covered in detail:



   Purification and determination of proteins

   Measuring enzymatic activity

   Microcalorimetry

   Immunoassays, affinity chromatography and other immunological methods

   Cross-linking, cleavage, and chemical modification of proteins

   Light microscopy, electron microscopy and atomic force microscopy

   Chromatographic and electrophoretic techniques

   Protein sequence and composition analysis

   Mass spectrometry methods

   Measuring protein-protein interactions

   Biosensors

   NMR and EPR of biomolecules

   Electron microscopy and X-ray structure analysis

   Carbohydrate and lipid analysis

   Analysis of posttranslational modifications

   Isolation and determination of nucleic acids

   DNA hybridization techniques

   Polymerase chain reaction techniques

   Protein sequence and composition analysis

   DNA sequence and epigenetic modification analysis

   Analysis of protein-nucleic acid interactions

   Analysis of sequence data

   Proteomics, metabolomics, peptidomics and toponomics

   Chemical biology