Bioluminescence- Methods and Protocols (Methods in Molecular Biology)

[ FreeCourseWeb.com ] Bioluminescence: Methods and Protocols (Methods in Molecular Biology)



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English | 320 pages | Humana Press; 1 edition (March 5, 1998) | 0896035204 | PDF | 17.93 Mb

A state-of-the-art collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protocols to a high degree of reproducibility-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods are also included for the construction of other recombinant organisms.



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